Clcn5^tm6.1Tjj
Targeted Allele Detail

Summary

• Symbol: Clcn5^tm6.1Tjj
• Name: chloride channel, voltage-sensitive 5; targeted mutation 6.1, Thomas J Jentsch
• MGI ID: MGI:4834486
• Synonyms: clcn5^lox
• Gene: Clcn5 Location: ChrX:7020049-7185597 bp, - strand Genetic Position: ChrX, 3.21 cM, cytoband F3-F4
• Alliance: Clcn5^tm6.1Tjj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164474
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Conditional ready, No functional change) Mutations: Insertion, Nucleotide substitutions Mutation details: A loxP site was inserted downstream of exon 9 and exon 11 was replaced with one containing nucleotide substitutions that result in the amino acid substitution of glutamic acid for tyrosine at position 672 (Y672E). An additional loxP site was inserted downstream of exon 12. An FRT flanked neo cassette inserted downstream of the modified exon 11 was removed by flp mediated recombination leaving exon 10, the modified exon 11, and exon 12 floxed. (J:164474)

Generation of the floxed Clcn5^tm6.1Tjj allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Clcn5 Mutation: 17 strains or lines available

References

• Original: J:164474 Rickheit G, et al., Role of ClC-5 in renal endocytosis is unique among ClC exchangers and does not require PY-motif-dependent ubiquitylation. J Biol Chem. 2010 Jun 4;285(23):17595-603
• All: 2 reference(s)