Hprt1^{tm31(Ple67-EGFP)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm31(Ple67-EGFP)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 31, Elizabeth M Simpson
• MGI ID: MGI:4834648
• Synonyms: FEV-EGFP, Hprt^{tm31(mEMS2784)Ems}, Ple67
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm31(Ple67-EGFP)Ems} page

Mutation origin

• Mutant Cell Lines: mEMS2784, mEMS2788, mEMS2789, mEMS2790
• Germline Transmission: Earliest citation of germline transmission: J:145689
• Parent Cell Line: mEMS1204 (ES Cell)
• Strain of Origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
• Project Collection: Pleiades Promoter Project

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion Vector Type: Not Applicable
• Mutation details: The Ple67-EGFP transgene (pEMS1401) was designed with the 2242 bp Ple67 minipromoter (FEV-D; derived from a subsection of the promoter from the human FEV (ETS oncogene family) (FEV) gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome.

  The promoter/regulatory regions of the human FEV gene directs expression of enhanced green fluorescent protein (EGFP) to neuronal cell bodies with strong staining in the nucleus. All raphe nuclei show EGFP positive cells: dorsal raphe, obscurus/magnus raphe and pallidus raphe. (J:144244, J:164356)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

Notes

Germ line transmission of mutant cell line mEMS2784 has been established.

References

• Original: J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009
• All: 3 reference(s)