Hprt1tm54(Ple233-EGFP)Ems
Targeted Allele Detail
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Symbol: |
Hprt1tm54(Ple233-EGFP)Ems |
Name: |
hypoxanthine phosphoribosyltransferase 1; targeted mutation 54, Elizabeth M Simpson |
MGI ID: |
MGI:4834789 |
Synonyms: |
Hprttm54(mEMS3251)Ems, Ple233, TNNT1-EFGP |
Gene: |
Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
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Alliance: |
Hprt1tm54(Ple233-EGFP)Ems page
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Allele Type: |
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Targeted (Reporter) |
Mutation: |
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Insertion
Vector Type: Not Applicable
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Mutation details: The Ple233-EGFP transgene (pEMS1437) was designed with the 1216 bp Ple233 minipromoter (TNNT1-C; derived from a subsection of the promoter and a downstream putative regulatory element from the human troponin T type 1 (skeletal, slow) [TNNT1] gene) upstream of a minimal F5 mutant-frt site, an enhanced green fluorescent protein (with mutated TAA->TTA stop), a nuclear localization signal, a second minimal frt site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprtb-m3 mutant locus on the X chromosome.
(J:144244, J:164356)
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View phenotypes and curated references for all genotypes (concatenated display).
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Germ line transmission of mutant cell line mEMS3251 has been established.
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Original: |
J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009; |
All: |
3 reference(s) |
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