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Tg(Car1-cre)5Flt
Transgene Detail
Summary
Symbol: Tg(Car1-cre)5Flt
Name: transgene insertion 5, James C Fleet
MGI ID: MGI:4835007
Synonyms: CAC5
Transgene: Tg(Car1-cre)5Flt  Location: unknown  
Alliance: Tg(Car1-cre)5Flt page
Transgene
origin
Strain of Origin:  C57BL/6J
Transgene
description
Transgene Type:    Transgenic (Recombinase)
Mutations:    Deletion, Insertion
 
Tg(Car1-cre)5Flt expression driven by 1 gene
 
Mutation detailsThe 10.6 kb colon-specific promoter and the share 2.5 kb erythroid/colon enhancer from the mouse Car1 gene were PCR amplified and cloned in front of the cre recombinase gene in a plasmid vector. The purified construct was used to generated transgenic mice. Two lines were selected for further analysis. Line 5 (CAC5 with 40 copies to the transgene insertion) was shown by PCR to have 10-fold higher transgene mRNA levels than line 6 (CAC6 having 20 copies). The mRNA was detectable only in the large intestine from cecum to distal colon. A low level of mRNA expression was detected in the liver, but no other tissues analyzed expressed the transgene. In 2022, the investigator reported that ~3-22 copies of the transgene are integrated on chromosome 8 (85,597,602-85,597,668 [GRCm38]) and resulted in a 64bp deletion of genomic region in between the two identified breakpoints. There are no genes annotated in this region [NCBI genome browser - GRCm38 - Chr8:85,597,400-85,597,800]. (J:164247)
Recombinase
activity
Activity:
 Tissue activity of this recombinase allele
Driver: Car1 (mouse)
Summary of all recombinase alleles driven by Car1.
 

Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Tumor Data
List all tumor models in MMHCdb carrying Tg(Car1-cre)5Flt
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
References
Original:  J:164247 Xue Y, et al., Generation of a transgenic mouse for colorectal cancer research with intestinal cre expression limited to the large intestine. Mol Cancer Res. 2010 Aug;8(8):1095-104
All:  8 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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last database update
12/10/2024
MGI 6.24
The Jackson Laboratory