Hprt1^{tm73(Ple142-lacZ)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm73(Ple142-lacZ)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 73, Elizabeth M Simpson
• MGI ID: MGI:4835011
• Synonyms: Hprt^{tm73(mEMS4751)Ems}, NR2E1-lacZ, Ple142
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm73(Ple142-lacZ)Ems} page

Mutation origin

• Mutant Cell Lines: mEMS4748, mEMS4749, mEMS4750, mEMS4751
• Germline Transmission: Earliest citation of germline transmission: J:145689
• Parent Cell Line: mEMS1202 (ES Cell)
• Strain of Origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
• Project Collection: Pleiades Promoter Project

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion Vector Type: Not Applicable
• Mutation details: The Ple142-lacZ transgene (bEMS86) was designed with the Ple142 MaxiPromoter (NR2E1-X; derived from the BAC RP11-144P8 that contains the whole (NR2E1) gene plus ~42 kb upstream and ~52 kb downstream, and also contains a portion of the SNX3 gene in the downstream sequence from the nuclear receptor subfamily 2, group E, member 1 (NR2E1) gene) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome. (J:144244, J:164356)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

Notes

Germ line transmission of mutant cell line mEMS4751 has been established.

References

• Original: J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009
• All: 6 reference(s)