Hprt1^{tm53(CAG-lacZ)Ems}
Targeted Allele Detail

Summary

• Symbol: Hprt1^{tm53(CAG-lacZ)Ems}
• Name: hypoxanthine phosphoribosyltransferase 1; targeted mutation 53, Elizabeth M Simpson
• MGI ID: MGI:4835013
• Synonyms: CAG-lacZ, Hprt^{tm53(mEMS3960)Ems}
• Gene: Hprt1 Location: ChrX:52077014-52110536 bp, + strand Genetic Position: ChrX, 29.31 cM, cytoband A6
• Alliance: Hprt1^{tm53(CAG-lacZ)Ems} page

Mutation origin

• Mutant Cell Line: mEMS3960
• Germline Transmission: Earliest citation of germline transmission: J:145689
• Parent Cell Line: mEMS1202 (ES Cell)
• Strain of Origin: (B6.129P2-Hprt1^b-m3/J x 129S-Gt(ROSA)26Sor^tm1Sor/J)F1
• Project Collection: Pleiades Promoter Project

Mutation description

• Allele Type: Targeted
• Mutation: Insertion Vector Type: Not Applicable
• Mutation details: The CAG-lacZ transgene (pEMS1488) was designed with the 1627 bp CAG promoter (a human cytomegalovirus enhancer, chicken beta-actin promoter and 5' UTR, and chicken beta-actin intron/rabbit beta-globin splice acceptor, all from the pDrive-CAG vector) upstream of a frt-flanked beta-galactosidase (lacZ) gene, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2). This construct was targeted as a single copy knockin to the Hprt^b-m3 mutant locus on the X chromosome. (J:144244, J:164356)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hprt1 Mutation: 1279 strains or lines available

Notes

Germ line transmission of mutant cell line mEMS3960 has been established.

References

• Original: J:145689 The Pleiades Promoter Project, Generation of reporter and cre-expressing targeted transgenic mice in the Hprt1 gene using human MiniPromoters that drive region- and cell-specific gene expression in the mouse brain. MGI Direct Data Submission. 2009
• All: 3 reference(s)