Runx1^{tm1.1(RUNX1*)Homy}
Targeted Allele Detail

Summary

• Symbol: Runx1^{tm1.1(RUNX1*)Homy}
• Name: runt related transcription factor 1; targeted mutation 1.1, Hiroaki Honda
• MGI ID: MGI:4835663
• Synonyms: Runx1^deltaIRES
• Gene: Runx1 Location: Chr16:92398354-92622962 bp, - strand Genetic Position: Chr16, 53.7 cM
• Alliance: Runx1^{tm1.1(RUNX1*)Homy} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164806
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Humanized sequence, Hypomorph, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A cellular IRES in the 5' UTR of exon 3, the coding region of exon 3 and part of intron 3 were replaced with a floxed complete human RUNX1 splice variant B cDNA and an FRT flanked neo cassette via homologous recombination. The neo cassette was removed by flp mediated recombination. Blotting analysis indicated protein expression in whole homozygous embryos at E13.5 is similar to wild-type controls. (J:164806)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx1 Mutation: 34 strains or lines available

References

• Original: J:164806 Nagamachi A, et al., A 5' untranslated region containing the IRES element in the Runx1 gene is required for angiogenesis, hematopoiesis and leukemogenesis in a knock-in mouse model. Dev Biol. 2010 Sep 15;345(2):226-36
• All: 2 reference(s)