Grhl2^tm1.1Jane
Targeted Allele Detail

Summary

• Symbol: Grhl2^tm1.1Jane
• Name: grainyhead like transcription factor 2; targeted mutation 1.1, Stephen M Jane
• MGI ID: MGI:4836381
• Gene: Grhl2 Location: Chr15:37233280-37363813 bp, + strand Genetic Position: Chr15, 14.84 cM, cytoband C
• Alliance: Grhl2^tm1.1Jane page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164805
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A lacZ gene and floxed neo cassette were inserted just after the codon 11 in exon 2 replacing part of exon 2 and all of intron 3 and exon 3 via homologous recombination. Cre mediated recombination removed the neo cassette. RT-PCR analysis confirmed the absence of expression in homozygous embryos. (J:164805)

Expression

In Mice Carrying this Mutation:	38 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Grhl2 Mutation: 41 strains or lines available

References

• Original: J:164805 Rifat Y, et al., Regional neural tube closure defined by the Grainy head-like transcription factors. Dev Biol. 2010 Sep 15;345(2):237-45
• All: 6 reference(s)