Grhl3^tm2.1Jane
Targeted Allele Detail

Summary

• Symbol: Grhl3^tm2.1Jane
• Name: grainyhead like transcription factor 3; targeted mutation 2.1, Stephen M Jane
• MGI ID: MGI:4836382
• Synonyms: Grhl3^2ki
• Gene: Grhl3 Location: Chr4:135269199-135300941 bp, - strand Genetic Position: Chr4, 67.76 cM
• Alliance: Grhl3^tm2.1Jane page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164805
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An 1808 bp cDNA fragment of the mouse Grhl2 gene was inserted such that the codon 12 of exon 2 of Grhl3 was fused to the corresponding codon of Grhl2. This insertion replaced part of exon 2 and all of intron 3 and exon 3. In addition a floxed neo cassette was inserted after the cDNA and then removed by Cre mediated recombination. RT-PCR analysis confirmed the expression of the fusion gene in homozygous and heterozygous mice and absence of expression of the endogenous gene in homozygous mice. (J:164805)

Expression

In Mice Carrying this Mutation:

14 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Grhl3 Mutation: 53 strains or lines available

References

• Original: J:164805 Rifat Y, et al., Regional neural tube closure defined by the Grainy head-like transcription factors. Dev Biol. 2010 Sep 15;345(2):237-45
• All: 2 reference(s)