Plau^tm1.1Bug
Targeted Allele Detail

Summary

• Symbol: Plau^tm1.1Bug
• Name: plasminogen activator, urokinase; targeted mutation 1.1, Thomas H Bugge
• MGI ID: MGI:4836576
• Synonyms: Plau^GFDhu, uPA^{Y23N-R28N-R30H-R31W}
• Gene: Plau Location: Chr14:20886728-20893453 bp, + strand Genetic Position: Chr14, 11.53 cM
• Alliance: Plau^tm1.1Bug page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164542
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 4 was replaced with one in which nucleotide substitutions (resulting in the amino acid substitutions Y23N, R28N, R30H, and R31W) abolish the ligand receptor interacting domain governed by the burial of the beta-hairpin of the growth factor domain (GFD). The overal structure and catalytic function of the protein is preserved. A floxed neo cassette inserted downstream of the modified exon 4 was removed by cre mediated recombination. (J:164542)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Plau Mutation: 29 strains or lines available

References

• Original: J:164542 Connolly BM, et al., Selective abrogation of the uPA-uPAR interaction in vivo reveals a novel role in suppression of fibrin-associated inflammation. Blood. 2010 Sep 2;116(9):1593-603
• All: 7 reference(s)