Mdm2^tm1.1Ypz
Targeted Allele Detail

Summary

• Symbol: Mdm2^tm1.1Ypz
• Name: transformed mouse 3T3 cell double minute 2; targeted mutation 1.1, Yangping Zhang
• MGI ID: MGI:4836988
• Synonyms: Mdm2^C305F
• Gene: Mdm2 Location: Chr10:117524780-117546663 bp, - strand Genetic Position: Chr10, 66.32 cM, cytoband C1-C3
• Alliance: Mdm2^tm1.1Ypz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164200
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Site-directed mutagenesis converted cysteine codon 303 (TGT) in exon 12 to phenylalanine (TTT) (p.C303F). Additional, silent, mutations were introduced in the next two downstream codons to introduce a diagnostic SpeI restriction site. A loxP site flanked neomycin resistance gene cassette that was inserted into intron 10 was removed through subsequent Cre-mediated recombination. The mutation disrupts binding of the encoded peptide to Smr3a (RPL5) and RPL11 gene products. (J:164200)

Tumor Data

List all tumor models in MMHCdb carrying Mdm2^tm1.1Ypz

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Mdm2 Mutation: 54 strains or lines available

References

• Original: J:164200 Macias E, et al., An ARF-independent c-MYC-activated tumor suppression pathway mediated by ribosomal protein-Mdm2 Interaction. Cancer Cell. 2010 Sep 14;18(3):231-43
• All: 11 reference(s)