Sp8^tm2Smb
Targeted Allele Detail

Summary

• Symbol: Sp8^tm2Smb
• Name: trans-acting transcription factor 8; targeted mutation 2, Sheila M Bell
• MGI ID: MGI:4837153
• Synonyms: Sp8^flox
• Gene: Sp8 Location: Chr12:118810064-118816311 bp, + strand Genetic Position: Chr12, 63.48 cM
• Alliance: Sp8^tm2Smb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:86196
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: An FRT flanked neomycin selection cassette and a 5' loxP site were inserted in intron 2. A second loxP site was placed downstream of the zinc finger domain and stop codon in the 3' UTR. The coding sequence of the gene was thus surrounded by loxP sites. Sp8^tm1Smb was derived from this allele. (J:86196)

Expression

In Mice Carrying this Mutation:	14 assay results 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Sp8 Mutation: 29 strains or lines available

References

• Original: J:86196 Bell SM, et al., Sp8 is crucial for limb outgrowth and neuropore closure. Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12195-200
• All: 16 reference(s)