Mir33^tm1.1Koho
Targeted Allele Detail

Summary

• Symbol: Mir33^tm1.1Koho
• Name: microRNA 33; targeted mutation 1.1, Koh Ono
• MGI ID: MGI:4837623
• Gene: Mir33 Location: Chr15:82082323-82082391 bp, + strand Genetic Position: Chr15, 38.54 cM
• Alliance: Mir33^tm1.1Koho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:165420
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: A floxed pgk-neo cassette replaced the pre microRNA sequence located in intron 16 of Srebf2. Cre mediated recombination removed the neo cassette. Western blot and RT-PCR analysis confirmed that deletion of the microRNA did not disrupt splicing or expression of Srebf2. (J:165420)

Generation of the Mir33^tm1.1Koho allele

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mir33 Mutation: 1 strain or line available

References

• Original: J:165420 Horie T, et al., MicroRNA-33 encoded by an intron of sterol regulatory element-binding protein 2 (Srebp2) regulates HDL in vivo. Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17321-6
• All: 8 reference(s)