Rad50^tm2.1Flv
Targeted Allele Detail

Summary

• Symbol: Rad50^tm2.1Flv
• Name: RAD50 double strand break repair protein; targeted mutation 2.1, Richard A Flavell
• MGI ID: MGI:4837633
• Synonyms: RHS4,5,7^-, RHS457
• Gene: Rad50 Location: Chr11:53540346-53598146 bp, - strand Genetic Position: Chr11, 31.98 cM, cytoband A5-B1
• Alliance: Rad50^tm2.1Flv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:165055
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

Allele Type: Targeted Mutations: Insertion, Intragenic deletion Mutation details: In ES cells containing Rad50^tm1Flv, which lacks DNaseI hypersensitive site 7 (RHS7), floxed neo cassette replaces DNaseI hypersensitive site 4 and 5 (RHS4,5) between exon 21 and exon 22. Cre mediated recombination removed the neo cassette leaving the remaining DNaseI hypersensitive site 6 (RHS6) in intron 21 and exons 22 through 24 floxed. Western blot analysis confirmed normal protein expression. (J:165055, J:196154)

Generation of the Rad50^tm2.1Flv allele

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rad50 Mutation: 53 strains or lines available

References

• Original: J:165055 Koh BH, et al., Th2 LCR is essential for regulation of Th2 cytokine genes and for pathogenesis of allergic asthma. Proc Natl Acad Sci U S A. 2010 Jun 8;107(23):10614-9
• All: 2 reference(s)