Ncoa6^tm3.1Jxu
Targeted Allele Detail

Summary

• Symbol: Ncoa6^tm3.1Jxu
• Name: nuclear receptor coactivator 6; targeted mutation 3.1, Jianming Xu
• MGI ID: MGI:4837690
• Synonyms: Ncoa6^null
• Gene: Ncoa6 Location: Chr2:155232585-155315741 bp, - strand Genetic Position: Chr2, 77.26 cM, cytoband H2
• Alliance: Ncoa6^tm3.1Jxu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:165054
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted upstream of exon 8 and a loxP site and FRT flanked neo cassette were inserted downstream of exon 8 via homologous recombination. Flp mediated recombination removed the neo cassette. Cre mediated recombination removed exon 8. The absence of protein expression was confirmed by western blot analysis on E11.5 embryonic extracts. (J:165054, J:189065)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ncoa6 Mutation: 101 strains or lines available

References

• Original: J:165054 Wang WL, et al., Lens fiber cell differentiation and denucleation are disrupted through expression of the N-terminal nuclear receptor box of NCOA6 and result in p53-dependent and p53-independent apoptosis. Mol Biol Cell. 2010 Jul 15;21(14):2453-68
• All: 2 reference(s)