Tg(tetO-NOS2,-lacZ)240iMhus
Transgene Detail

Summary

• Symbol: Tg(tetO-NOS2,-lacZ)240iMhus
• Name: transgene insertion 240i, Mansoor Husain
• MGI ID: MGI:4837700
• Synonyms: iNOSBgal
• Transgene: Tg(tetO-NOS2,-lacZ)240iMhus Location: unknown
• Alliance: Tg(tetO-NOS2,-lacZ)240iMhus page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-NOS2,-lacZ)240iMhus expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  NOS2 (4843)

  Nos2	(MGI:97361)

• Mutation details: The tetO-NOS2 transgene was designed with a bidirectional tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator), pBi-1, between two cytomegalovirus (CMV) minimal promoters in the opposite orientation. One CMV promoter drives expression of the human nitric oxide synthase 2, inducible (NOS2 or iNOS) gene, followed by a simian virus 40 (SV40) polyadenylation (polyA) signal, while the other drives expression of a beta-galactosidase (lacZ) reporter followed by a beta-globin polyA signal. Lines 240i, 365iA, 365iB, and 365iC were generated. (J:132082)

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:132082 Mungrue IN, et al., Cardiomyocyte overexpression of iNOS in mice results in peroxynitrite generation, heart block, and sudden death. J Clin Invest. 2002 Mar;109(6):735-43
• All: 1 reference(s)