Tg(tetO-EDN1,-lacZ)9Mhus
Transgene Detail

Summary

• Symbol: Tg(tetO-EDN1,-lacZ)9Mhus
• Name: transgene insertion 9, Mansoor Husain
• MGI ID: MGI:4837732
• Synonyms: ET-1
• Transgene: Tg(tetO-EDN1,-lacZ)9Mhus Location: unknown
• Alliance: Tg(tetO-EDN1,-lacZ)9Mhus page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Humanized sequence, Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutation: Insertion
• Tg(tetO-EDN1,-lacZ)9Mhus expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  EDN1 (1906)

  Edn1	(MGI:95283)

• Mutation details: The tetO-EDN1 transgene was designed with a bidirectional tetracycline operator (tetO; also called tetracycline-responsive element (TRE, TetRE) or tet-operator), pBi-1, between two cytomegalovirus (CMV) minimal promoters in the opposite orientation. One CMV promoter drives expression of the human endothelin (EDN1 or ET-1) gene, followed by a simian virus 40 (SV40) polyadenylation (polyA) signal, while the other drives expression of a beta-galactosidase (lacZ) reporter followed by a beta-globin polyA signal. Three lines were generated (9, 101, and 403). Line 9 exhibits high levels of expression in the myocardium. (J:131245)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:131245 Yang LL, et al., Conditional cardiac overexpression of endothelin-1 induces inflammation and dilated cardiomyopathy in mice. Circulation. 2004 Jan 20;109(2):255-61
• All: 4 reference(s)