Igs2^{tm1(ACTB-EGFP,-tdTomato)Luo}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm1(ACTB-EGFP,-tdTomato)Luo}
• Name: intergenic site 2; targeted mutation 1, Liqun Luo
• MGI ID: MGI:4837736
• Synonyms: GT: GFP^N-termtdT^C-term, Iis2^{tm1(ACTB-EGFP,-tdTomato)Luo}, MADM-11-GT, MADM-11^GT, Tg(ACTB-EGFP,-tdTomato)11Luo
• Gene: Igs2 Location: unknown Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm1(ACTB-EGFP,-tdTomato)Luo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166047
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The GT MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 1-273), a beta-globin intronic sequence containing an frt site and loxP-flanked neomycin resistance gene, the C-terminal portion of a red fluorescent protein (tdTomato; aa 4-1544) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal. This entire GT MADM construct was inserted into the "Hipp11" locus on chromosome 11 (cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci). (J:166047)

Tumor Data

List all tumor models in MMHCdb carrying Igs2^{tm1(ACTB-EGFP,-tdTomato)Luo}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 72 strains or lines available

References

• Original: J:166047 Hippenmeyer S, et al., Genetic mosaic dissection of lis1 and ndel1 in neuronal migration. Neuron. 2010 Nov 18;68(4):695-709
• All: 39 reference(s)