Igs2^{tm2(ACTB-tdTomato,-EGFP)Luo}
Targeted Allele Detail

Summary

• Symbol: Igs2^{tm2(ACTB-tdTomato,-EGFP)Luo}
• Name: intergenic site 2; targeted mutation 2, Liqun Luo
• MGI ID: MGI:4837863
• Synonyms: Iis2^{tm2(ACTB-tdTomato,-EGFP)Luo}, MADM-11-TG, MADM-11^TG, Tg(ACTB-tdTomato,-EGFP)11Luo, TG: tdT^N-termGFP^C-term
• Gene: Igs2 Location: Chr11:3195460-3195497 bp Genetic Position: Chr11, Syntenic
• Alliance: Igs2^{tm2(ACTB-tdTomato,-EGFP)Luo} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166047
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The TG MADM targeting construct was designed with a CMV enhancer/chicken beta-actin core promoter (pCA), the N-terminal portion of a red fluorescent protein (tdTomato; aa 1-3), a beta-globin intronic sequence containing an frt site and loxP-flanked neomycin resistance gene, the C-terminal portion of a mutant enhanced green fluorescent protein (mut4-EGFP; aa 274-724), and an SV40 T-antigen poly(A) signal. This entire TG MADM construct was inserted into the "Hipp11" locus on chromosome 11 (cytoband A1 at ~3cM between the Eif4enif1 and Drg1 loci). (J:166047)

Tumor Data

List all tumor models in MMHCdb carrying Igs2^{tm2(ACTB-tdTomato,-EGFP)Luo}

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Igs2 Mutation: 72 strains or lines available

References

• Original: J:166047 Hippenmeyer S, et al., Genetic mosaic dissection of lis1 and ndel1 in neuronal migration. Neuron. 2010 Nov 18;68(4):695-709
• All: 39 reference(s)