Hand1^{tm1.1(EGFP/cre)Abfi}
Targeted Allele Detail

Summary

• Symbol: Hand1^{tm1.1(EGFP/cre)Abfi}
• Name: heart and neural crest derivatives expressed 1; targeted mutation 1.1, Anthony B Firulli
• MGI ID: MGI:4838016
• Synonyms: Hand1^{EGFPCredeltaNeo}
• Gene: Hand1 Location: Chr11:57719539-57722973 bp, - strand Genetic Position: Chr11, 35.27 cM
• Alliance: Hand1^{tm1.1(EGFP/cre)Abfi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:165398
• Parent Cell Line: CCE916 (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutations: Insertion, Intragenic deletion
• Hand1^{tm1.1(EGFP/cre)Abfi} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Hand1 (MGI:103577)

• Mutation details: A targeting vector containing a 3.4 kb EGFP/cre cassette with a 3' Frt-flanked neomycin cassette was introduced into the 5' untranslated region of Hand1. Homologous recombination resulted in deletion of the first exon of Hand1, producing a null allele, and insertion of the EGFP/cre gene under control of the endogenous promoter. Following germline transmission, the neo cassette was deleted by crossing mice to FLPeR homozygous mice. (J:165398)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Hand1 (mouse) Summary of all recombinase alleles driven by Hand1. Comparative matrix view of recombinase activities Alliance mouse Hand1 gene page

Expression

In Mice Carrying this Mutation:

16 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Hand1 Mutation: 15 strains or lines available

References

• Original: J:165398 Barnes RM, et al., Analysis of the Hand1 cell lineage reveals novel contributions to cardiovascular, neural crest, extra-embryonic, and lateral mesoderm derivatives. Dev Dyn. 2010 Nov;239(11):3086-97
• All: 6 reference(s)