App^tm2.1Zhe
Targeted Allele Detail

Summary

• Symbol: App^tm2.1Zhe
• Name: amyloid beta precursor protein; targeted mutation 2.1, Hui Zheng
• MGI ID: MGI:4838049
• Synonyms: App sbeta KI
• Gene: App Location: Chr16:84751236-84972187 bp, - strand Genetic Position: Chr16, 46.92 cM, cytoband C3-qter
• Alliance: App^tm2.1Zhe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:165417
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The mutated allele contains a loxP site immediately upstream of an exon 16 sequence modified by the insertion of a FLAG motif and a stop codon just downstream of the beta secretase cleavage site. The mutated copy of exon 16 was separated from a second, intact, copy of exon 16 by a sequence cassette containing two copies of neomycin flanked by FRT sites and followed by a second loxP site. Flp recombinase was then used to excise the two neomycin sequences leaving the mutated exon 16 surrounded by loxP sites. Transmembrane and intracellular sequences are deleted from the translation product. (J:165417)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any App Mutation: 105 strains or lines available

References

• Original: J:165417 Li H, et al., Soluble amyloid precursor protein (APP) regulates transthyretin and Klotho gene expression without rescuing the essential function of APP. Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17362-7
• All: 2 reference(s)