Tg(Alb1-GP33/H2-K^b)1Hpi
Transgene Detail

Summary

• Symbol: Tg(Alb1-GP33/H2-K^b)1Hpi
• Name: transgene insertion 1, Hanspeter Pircher
• MGI ID: MGI:4838097
• Transgene: Tg(Alb1-GP33/H2-K^b)1Hpi Location: unknown
• Alliance: Tg(Alb1-GP33/H2-K^b)1Hpi page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: The mouse albumin promoter fused to the promoterless genomic H-2Kb gene, in which the signal sequence of the Kb molecule encoded by exon 1 was replaced by the leader sequence (aa 1-60) of the LCMV glycoprotein (GP) encoding the GP33 epitope (aa 33-41). The construct does not encode a functional Kb molecule, since insertion of the LCMV GP fragment leads to a frame shift and a stop codon in the second exon. RT-PCR analysis revealed GP33 transgene expression in the thymus and liver (100-1000x the level of the thymus). (J:162605)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:162605 Voehringer D, et al., Break of T cell ignorance to a viral antigen in the liver induces hepatitis. J Immunol. 2000 Sep 1;165(5):2415-22
• All: 1 reference(s)