Pdpk1^tm1.1Daca
Targeted Allele Detail

Summary

• Symbol: Pdpk1^tm1.1Daca
• Name: 3-phosphoinositide dependent protein kinase 1; targeted mutation 1.1, Doreen A Cantrell
• MGI ID: MGI:4838167
• Synonyms: PDK1^K111A
• Gene: Pdpk1 Location: Chr17:24292654-24369898 bp, - strand Genetic Position: Chr17, 12.23 cM, cytoband A3.3
• Alliance: Pdpk1^tm1.1Daca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:165626
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Exon 4 was replaced with one in which nucleotide substitution resulted in the amino acid substitution of alanine for lysine at position 111 (K111A) and a neo cassette. Cre mediated recombination removed the neo cassette. The absence of catalytic activity was confirmed in E11.5 to E12.5 embryo lysates despite confirmed protein expression. (J:165626)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pdpk1 Mutation: 138 strains or lines available

References

• Original: J:165626 Finlay DK, et al., Temporal Differences in the Dependency on Phosphoinositide-Dependent Kinase 1 Distinguish the Development of Invariant V{alpha}14 NKT Cells and Conventional T Cells. J Immunol. 2010 Nov 15;185(10):5973-82
• All: 1 reference(s)