Wnk1^tm1.1Juha
Targeted Allele Detail

Summary

• Symbol: Wnk1^tm1.1Juha
• Name: WNK lysine deficient protein kinase 1; targeted mutation 1.1, Juliette Hadchouel
• MGI ID: MGI:4838243
• Synonyms: KS-WNK1^-
• Gene: Wnk1 Location: Chr6:119900930-120015633 bp, - strand Genetic Position: Chr6, 56.86 cM
• Alliance: Wnk1^tm1.1Juha page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:165537
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

Allele Type: Targeted (Hypomorph, Modified isoform(s)) Mutations: Insertion, Intragenic deletion Mutation details: A loxP site was inserted upstream of exon 4, an FRT site was inserted upstream of exon 4a, and a neo cassette flanked by attP and attB with 3' FRT and loxP site was inserted downstream of exon 4a. Flp mediated recombination removed the neo cassette and exon 4a. Exon 4 remains floxed. Real-time RT-PCR confirms the absence of the kidney specific transcript isoform. (J:165537)

Generation of the Wnk1^tm1.1Juha allele

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wnk1 Mutation: 115 strains or lines available

References

• Original: J:165537 Hadchouel J, et al., Decreased ENaC expression compensates the increased NCC activity following inactivation of the kidney-specific isoform of WNK1 and prevents hypertension. Proc Natl Acad Sci U S A. 2010 Oct 19;107(42):18109-14
• All: 3 reference(s)