Gt(ROSA)26Sor^{tm1(SRF/VP16)Antu}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(SRF/VP16)Antu}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Alfred Nordheim
• MGI ID: MGI:4838411
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(SRF/VP16)Antu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:172655
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(SRF/VP16)Antu} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  SRF (6722)

  Srf	(MGI:106658)

• Mutation details: A C-terminally truncated human SRF cDNA encoding amino acid residues 1-412 was fused to the transcription activation domain of the herpes simplex virus protein VP16. This was preceeded by a splice acceptor site, a PGK-puromycin selection cassette, and a stop cassette composed of four SV40 polyadenylation signals. The selection cassette and stop cassette were flanked by loxP sites. This construct was then inserted following exon I of the endogenous gene. Despite the requirement of cre recombination to removed the stop cassette and achieve full SRF/VP16 expression, some leaky read-through transcript expression is detected in the retina. (J:172655)

Expression

In Structures Affected by this Mutation:

10 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 993 strains or lines available

References

• Original: J:170074 Sandstrom J, et al., Submission in advance of publication in Molecular Vision. MGI Direct Data Submission. 2011
• All: 3 reference(s)