Mcm10^{tm1(NCOM)Cmhd}
Targeted Allele Detail

Summary

• Symbol: Mcm10^{tm1(NCOM)Cmhd}
• Name: minichromosome maintenance 10 replication initiation factor; targeted mutation 1, Centre for Modelling Human Disease
• MGI ID: MGI:4839016
• Gene: Mcm10 Location: Chr2:4995535-5017602 bp, - strand Genetic Position: Chr2, 3.12 cM, cytoband A1
• Alliance: Mcm10^{tm1(NCOM)Cmhd} page
• IMPC: Mcm10 gene page

Mutation origin

• Mutant Cell Lines: N00006P0_C_6T_A5, N00006P0_C_6T_B5, N00006P0_C_6T_C5, N00006P0_C_6T_D5, N00006P0_C_6T_E5, N00006P0_C_6T_F5, N00006P0_C_6T_G5, N00006P0_C_6T_H5
• Germline Transmission: Earliest citation of germline transmission: J:165965
• Parent Cell Line: C2 (Tcp) (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: NorCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_NTARU-1
• Mutation details: The insertion of NorCOMM cassette pNTARU created a deletion of size 436bp between positions 5011054-5011490 of Chromosome 2 (Build GRCm39). This deletion results in the removal of functionally critical exon(s). The cassette is composed of an F3 site followed by a universal inverse PCR primer site and a splice acceptor lacZ-neomycin phosphotransferase fusion (beta-geo) sequence. This is followed by a PhiC31 attP site and a puromycin sequence that is not active unless a promoter is inserted by PhiC31 at the attP site. The cassette is finished with an FRT site. Variations of this allele can be made using PhiC31 docking and subsequent cloning (Nagy et al., 2009. Methods Mol. Biol. 530:365-78). (J:165963)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Mcm10 Mutation: 55 strains or lines available

References

• Original: J:165963 Centre for Modeling Human Disease, Alleles produced for the NorCOMM project by the Centre for Modeling Human Disease (Cmhd), Institute of Biomaterials & Biomedical Engineering, University of Toronto. MGI Direct Data Submission. 2010
• All: 3 reference(s)