Fbn1^tm1.2Lysa
Targeted Allele Detail

Summary

• Symbol: Fbn1^tm1.2Lysa
• Name: fibrillin 1; targeted mutation 1.2, Lynn Y Sakai
• MGI ID: MGI:4839045
• Synonyms: GT8
• Gene: Fbn1 Location: Chr2:125142514-125348417 bp, - strand Genetic Position: Chr2, 61.38 cM, cytoband F
• Alliance: Fbn1^tm1.2Lysa page

Fragmentation of aortic root elastic lamellae in Fbn1^tm1.2Lysa/Fbn1⁺ mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:165897
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Inversion Fbn1^tm1.2Lysa involves 1 genes/genome features (Fbn1) View all Mutation details: A lox66 site was inserted upstream of exon 33. The following vector was inserted into intron 34 (5' through 3'): eGFP, exon 33 splice acceptor site, lox77 site, and FRT flanked neo cassette. Flp mediated recombination removed the neo cassette. Cre mediated recombination inverted the region between lox66 and lox77, bringing eGFP into frame after exon 32. The predicted product is truncated after the 17th calcium-binding epidermal growth factor-like domain (cbEGF17) and called GT8 ("green truncated" from founder mouse 8). (J:165897)

Generation of the Fbn1^tm1.2Lysa allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Fbn1 Mutation: 173 strains or lines available

References

• Original: J:165897 Charbonneau NL, et al., In vivo studies of mutant fibrillin-1 microfibrils. J Biol Chem. 2010 Aug 6;285(32):24943-55
• All: 4 reference(s)