Zan^tm1Dmh
Targeted Allele Detail

Summary

• Symbol: Zan^tm1Dmh
• Name: zonadhesin; targeted mutation 1, Daniel M Hardy
• MGI ID: MGI:4839099
• Gene: Zan Location: Chr5:137376899-137475326 bp, - strand Genetic Position: Chr5, 76.4 cM
• Alliance: Zan^tm1Dmh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:165895
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

Allele Type: Targeted (Null/knockout) Mutations: Insertion, Intragenic deletion Mutation details: The proximal promoter and first 6 exons were replaced with a floxed neo cassette. The absence of protein expression was confirmed by western blot analysis and indirect immunofluorescence on spermatozoa. (J:165895)

Generation of the Zan^tm1Dmh allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Zan Mutation: 220 strains or lines available

References

• Original: J:165895 Tardif S, et al., Zonadhesin is essential for species specificity of sperm adhesion to the egg zona pellucida. J Biol Chem. 2010 Aug 6;285(32):24863-70
• All: 2 reference(s)