Med12^tm1.2Hsch
Targeted Allele Detail

Summary

• Symbol: Med12^tm1.2Hsch
• Name: mediator complex subunit 12; targeted mutation 1.2, Heinrich Schrewe
• MGI ID: MGI:4839486
• Synonyms: Med12^delta1-7
• Gene: Med12 Location: ChrX:100317636-100341071 bp, + strand Genetic Position: ChrX, 43.91 cM, cytoband C2
• Alliance: Med12^tm1.2Hsch page

Mutation origin

• Mouse Generated: Earliest citation of chimera generation: J:166040
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: One loxP site was inserted in the 5'UTR of exon 1 while a second loxP site, followed by an FRT flanked neomycin cassette in reverse orientation, was inserted in intron 7. The selection cassette was subsequently removed by transient flp recombinase expression leaving exons 1-7 flanked by loxP sites. Transient cre expression then excised the floxed exons. (J:166040)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Med12 Mutation: 6 strains or lines available

References

• Original: J:166040 Rocha PP, et al., Med12 is essential for early mouse development and for canonical Wnt and Wnt/PCP signaling. Development. 2010 Aug;137(16):2723-31
• All: 2 reference(s)