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Gt(ROSA)26Sortm7(SMO*/YFP)Amc
Targeted Allele Detail
Summary
Symbol: Gt(ROSA)26Sortm7(SMO*/YFP)Amc
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 7, Andrew P McMahon
MGI ID: MGI:4839792
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance: Gt(ROSA)26Sortm7(SMO*/YFP)Amc page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:165962
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  mixed
Mutation
description
Allele Type:    Targeted (Conditional ready, Inserted expressed sequence, Reporter)
Mutation:    Insertion
 
Gt(ROSA)26Sortm7(SMO*/YFP)Amc expresses 1 gene
 
Mutation detailsA tetracycline regulatory unit and polyadenylation signals were inserted upstream of a tetO and minimal CMV promoter. This was followed by a floxed PGK-neomycin-polyA cassette and then by a cDNA for the human smoothened homolog containing a Trp to Leu mutation in codon 535 and fused to YFP. Expression of the SMO* knock-in occurs only after cre excision of the PGK-neo cassette which can be accomplished by tamoxifen treatment to activate cre expression from the TG(CAG-cre/Esr1*)5Amc transgene carried by the YFP3-1 ES cell line used to create this mutation. (J:165962)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  992 strains or lines available
Notes
YFP3-1 ES cell line = YFP3-1. These cells were derived from embryos resulting from an intercross between Gt(ROSA)26Sortm1Sor; Tg(CAG-cre/Esr1*)5Amc male mice and female Gt(ROSA)26Sortm1(EYFP)Cos mice. This male ES cell line carries Tg(CAG-cre/Esr1*)5Amc and is heterozygous Gt(ROSA)26Sortm1Sor/tm1(EYFP)Cos on a background involving 129S4/SvJae, 129X1/SvJ, C57BL/6, CBA, and Swiss Webster.
References
Original:  J:165962 Mao J, et al., An ES cell system for rapid, spatial and temporal analysis of gene function in vitro and in vivo. Nucleic Acids Res. 2005;33(18):e155
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory