Chst15^tm1Oh
Targeted Allele Detail

Summary

• Symbol: Chst15^tm1Oh
• Name: carbohydrate sulfotransferase 15; targeted mutation 1, Osami Habuchi
• MGI ID: MGI:4840254
• Synonyms: Galnac4s-6st^-
• Gene: Chst15 Location: Chr7:131837509-131918957 bp, - strand Genetic Position: Chr7, 76.29 cM, cytoband F4
• Alliance: Chst15^tm1Oh page

Abnormal morphology of Chst15^tm1Oh/Chst15^tm1Oh bone marrow-derived mast cells

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:165927
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2 which contains the 3'-phosphoadenosine 5'-phosphosulfate binding site critical for sulfotransferase activity was replaced with a PGK-neomycin selection cassette. RT-Pcr of spleen RNA demonstrated the absence of expression. (J:165927)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Chst15 Mutation: 24 strains or lines available

References

• Original: J:165927 Ohtake-Niimi S, et al., Mice deficient in N-acetylgalactosamine 4-sulfate 6-o-sulfotransferase are unable to synthesize chondroitin/dermatan sulfate containing N-acetylgalactosamine 4,6-bissulfate residues and exhibit decreased protease activity in bone marrow-derived mast cells. J Biol Chem. 2010 Jul 2;285(27):20793-805
• All: 2 reference(s)