Sema4c^tm1Matl
Targeted Allele Detail

Summary

• Symbol: Sema4c^tm1Matl
• Name: sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C; targeted mutation 1, Marc Tessier-Lavigne
• MGI ID: MGI:4840317
• Gene: Sema4c Location: Chr1:36587720-36597430 bp, - strand Genetic Position: Chr1, 15.32 cM, cytoband B
• Alliance: Sema4c^tm1Matl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:167973
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector
• Mutation details: A secretory trap cassette was inserted by gene targeting between exons 12 and 13 of the Sema4C gene (exon 1 counted as the exon containing the start codon), which is upstream of the exons encoding the transmembrane (TM) and intracellular domains of Sema4C. The bicistronic mutant transcript encodes two proteins: a fusion of the extracellular domain of the Sema4C protein to the TM and beta-geo domains of the trap vector, and placental alkaline phosphatase (PLAP). Fusion proteins of endogenous proteins with the TM-beta-geo domain of the secretory trap vector, such as the Sema4C-TM-beta-geo fusion, are retained intracellularly and are not present on the cell surface, thereby generating functional null mutations. The absence of protein expression was confirmed by western blot analysis on cerebellar extracts. (J:167973)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sema4c Mutation: 36 strains or lines available

References

• Original: J:167973 Maier V, et al., Semaphorin 4C and 4G are ligands of Plexin-B2 required in cerebellar development. Mol Cell Neurosci. 2011 Feb;46(2):419-31
• All: 7 reference(s)