Rec8^tm1.1Kktk
Targeted Allele Detail

Summary

• Symbol: Rec8^tm1.1Kktk
• Name: REC8 meiotic recombination protein; targeted mutation 1.1, Kikue Tachibana-Konwalski
• MGI ID: MGI:4840466
• Synonyms: Rec8^e10TEV, Rec8^TEV
• Gene: Rec8 Location: Chr14:55855494-55862852 bp, + strand Genetic Position: Chr14, 28.19 cM
• Alliance: Rec8^tm1.1Kktk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166151
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Three consecutive TEV protease recognition sites with a novel MfeI restriction site were introduced between amino acids S274 and P275 in exon 10. A floxed puro cassette was inserted downstream of exon 11. Cre mediated recombination removed the puro cassette. (J:166151)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rec8 Mutation: 25 strains or lines available

References

• Original: J:166151 Tachibana-Konwalski K, et al., Rec8-containing cohesin maintains bivalents without turnover during the growing phase of mouse oocytes. Genes Dev. 2010 Nov 15;24(22):2505-16
• All: 4 reference(s)