Rad21^tm1.1Kktk
Targeted Allele Detail

Summary

• Symbol: Rad21^tm1.1Kktk
• Name: RAD21 cohesin complex component; targeted mutation 1.1, Kikue Tachibana-Konwalski
• MGI ID: MGI:4840469
• Synonyms: Scc1e^11TEVMyc, Scc1^TEVMyc
• Gene: Rad21 Location: Chr15:51825636-51855143 bp, - strand Genetic Position: Chr15, 19.54 cM, cytoband D3
• Alliance: Rad21^tm1.1Kktk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166151
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: Three consecutive TEV protease recognition sites with a novel BamHI restriction site were inserted between P475 and P476 of exon 11. At the stop codon an additional BamHI was inserted as well as a C-terminal c-Myc epitope tag with a stop codon. A floxed puro cassette was inserted downstream of exon 12. Cre mediated recombination removed the puro cassette. (J:166151)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rad21 Mutation: 60 strains or lines available

References

• Original: J:166151 Tachibana-Konwalski K, et al., Rec8-containing cohesin maintains bivalents without turnover during the growing phase of mouse oocytes. Genes Dev. 2010 Nov 15;24(22):2505-16
• All: 4 reference(s)