Daam2^{tm1a(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Daam2^{tm1a(KOMP)Wtsi}
• Name: dishevelled associated activator of morphogenesis 2; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4841108
• Gene: Daam2 Location: Chr17:49763050-49871371 bp, - strand Genetic Position: Chr17, 25.5 cM
• Alliance: Daam2^{tm1a(KOMP)Wtsi} page
• IMPC: Daam2 gene page

Mutation origin

• Mutant Cell Lines: EPD0660_3_A09, EPD0660_3_B10, EPD0660_3_B12, EPD0660_3_C12, EPD0660_3_E09, EPD0660_3_E10, EPD0660_3_E11, EPD0660_3_F09, EPD0660_3_F10, EPD0660_3_F11, EPD0660_3_G10, EPD0660_3_H09, EPD0660_3_H10, EPD0660_3_H11
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Pgk_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 49796759 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 49797839. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:148605, J:173534)

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 1 line available
• Carrying any Daam2 Mutation: 50 strains or lines available

Notes

Cell line EPD0660_3_A09 was successfully used to make chimeric mice. Germline transmission was accomplished. J:204986

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 14 reference(s)