Hacd2^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Hacd2^{tm1a(EUCOMM)Hmgu}
• Name: 3-hydroxyacyl-CoA dehydratase 2; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4841604
• Synonyms: Hacd2^flox, Hacd2^KD
• Gene: Hacd2 Location: Chr16:34842798-34929547 bp, + strand Genetic Position: Chr16, 24.54 cM
• Alliance: Hacd2^{tm1a(EUCOMM)Hmgu} page
• IMPC: Hacd2 gene page

Mutation origin

• Mutant Cell Lines: HEPD0663_6_A10, HEPD0663_6_B09, HEPD0663_6_B12, HEPD0663_6_C12, HEPD0663_6_D09, HEPD0663_6_D10, HEPD0663_6_E11, HEPD0663_6_F09, HEPD0663_6_F12, HEPD0663_6_G09
• Germline Transmission: Earliest citation of germline transmission: J:333944
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 34845737 of Chromosome 16 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 34846568. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Hacd2 Mutation: 18 strains or lines available

Notes

Cell line HEPD0663_6_E11 was not successfully used to make chimeric mice. Germline transmission was not accomplished. J:333944

Cell lines HEPD0663_6_A10 and HEPD0663_6_G09 were successfully used to make chimeric mice. Germline transmission was accomplished. J:333944

Special note about mutant cell lines HEPD0663_6_A10 and HEPD0663_6_G09: The mutation generated in HEPD0663_6_A10 or HEPD0663_6_G09 results in a hypomorphic allele. RT-qPCR analysis confirmed reduced levels of mRNA in homozygous mutant pups, with the strongest reduction observed in liver (- 88%), kidney (- 84%), BAT (- 85%) and heart (- 83%), and a less pronounced reduction observed in skeletal muscle (- 54%).

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)