Rtca^{tm1a(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Rtca^{tm1a(EUCOMM)Hmgu}
• Name: RNA 3'-terminal phosphate cyclase; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:4841792
• Synonyms: Rtca^Ins, Rtca^LacZ_loxP
• Gene: Rtca Location: Chr3:116282612-116301852 bp, - strand Genetic Position: Chr3, 50.35 cM
• Alliance: Rtca^{tm1a(EUCOMM)Hmgu} page
• IMPC: Rtca gene page

Mutation origin

• Mutant Cell Lines: HEPD0685_1_A10, HEPD0685_1_B10, HEPD0685_1_B11, HEPD0685_1_B12, HEPD0685_1_C10, HEPD0685_1_D10, HEPD0685_1_E10, HEPD0685_1_F10, HEPD0685_1_G10, HEPD0685_1_G12, HEPD0685_1_H10, HEPD0685_1_H11
• Germline Transmission: Earliest citation of germline transmission: J:233466
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 116297780 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 116298701. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157065)

Expression

In Mice Carrying this Mutation:	6 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Rtca Mutation: 20 strains or lines available

Notes

Special note about the mutation generated in J:233466: Western blot analysis of retinal tissue revealed a ~82% reduction of full-length protein in homozygous mutant mice, suggesting that this is a hypomorphic allele.

References

• Original: J:157065 Helmholtz Zentrum Muenchen GmbH, Alleles produced for the EUCOMM and EUCOMMTools projects by the Helmholtz Zentrum Muenchen GmbH (Hmgu). MGI Direct Data Submission. 2010-2015
• All: 2 reference(s)