Fbxo7^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Fbxo7^{tm1a(EUCOMM)Wtsi}
• Name: F-box protein 7; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4842259
• Gene: Fbxo7 Location: Chr10:85857836-85887737 bp, + strand Genetic Position: Chr10, 41.91 cM
• Alliance: Fbxo7^{tm1a(EUCOMM)Wtsi} page
• IMPC: Fbxo7 gene page

Mutation origin

• Mutant Cell Lines: EPD0622_3_A01, EPD0622_3_A04, EPD0622_3_A05, EPD0622_3_B02, EPD0622_3_B03, EPD0622_3_B04, EPD0622_3_B05, EPD0622_3_B06, EPD0622_3_C03, EPD0622_3_C05, EPD0622_3_D01, EPD0622_3_D02, EPD0622_3_D04, EPD0622_3_E02, EPD0622_3_E03, EPD0622_3_F03, EPD0622_3_F04, EPD0622_3_F05, EPD0622_3_G01, EPD0622_3_G02, EPD0622_3_G03, EPD0622_3_G06, EPD0622_3_H01, EPD0622_3_H05
• Germline Transmission: Earliest citation of germline transmission: J:175295
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 85866305 of Chromosome 10 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 85867153. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 1 line available
• Carrying any Fbxo7 Mutation: 27 strains or lines available

Notes

Special note about mutant cell line EPD0622_3_D02: The mutation generated in this ES cell line results in a hypomorphic allele with 4-16% residual Fbxo7 transcript depending on the tissue tested, indicating incomplete ablation of gene expression (J:272313).

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 7 reference(s)