Dhpstm2a(EUCOMM)Wtsi
Targeted Allele Detail
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Symbol: |
Dhpstm2a(EUCOMM)Wtsi |
Name: |
deoxyhypusine synthase; targeted mutation 2a, Wellcome Trust Sanger Institute |
MGI ID: |
MGI:4842333 |
Synonyms: |
Dhstm1a |
Gene: |
Dhps Location: Chr8:85798386-85801791 bp, + strand Genetic Position: Chr8, 41.55 cM
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Alliance: |
Dhpstm2a(EUCOMM)Wtsi page
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IMPC: |
Dhps gene page |
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Mutant Cell Lines: |
EPD0628_1_B06, EPD0628_1_B08, EPD0628_1_C06, EPD0628_1_F05 |
Germline Transmission: |
Earliest citation of germline transmission:
J:204812
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Parent Cell Line: |
JM8A3.N1 (ES Cell)
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Strain of Origin: |
C57BL/6N-Atm1Brd
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Project Collection: |
EUCOMM
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Allele Type: |
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Targeted (Conditional ready, Null/knockout, Reporter) |
Mutation: |
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Insertion
Vector: L1L2_Bact_P
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Mutation details: The L1L2_Bact_P cassette was inserted at position 85798794 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 85801230. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:155845, J:173534)
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View phenotypes and curated references for all genotypes (concatenated display).
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Cell line EPD0628_1_B06, EPD0628_1_C06 and EPD0628_1_F05 were successfully used to make chimeric mice. Germline transmission was accomplished. J:236582
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Original: |
J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010; |
All: |
6 reference(s) |
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