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Katnal2tm1a(EUCOMM)Wtsi
Targeted Allele Detail
Summary
Symbol: Katnal2tm1a(EUCOMM)Wtsi
Name: katanin p60 subunit A-like 2; targeted mutation 1a, Wellcome Trust Sanger Institute
MGI ID: MGI:4842422
Synonyms: Katnal2KO
Gene: Katnal2  Location: Chr18:77064844-77135004 bp, - strand  Genetic Position: Chr18, 52.02 cM, cytoband E3
Alliance: Katnal2tm1a(EUCOMM)Wtsi page
IMPC: Katnal2 gene page
Spermiation and spermatid defects in Katnal2m1Anu/Katnal2tm1a(EUCOMM)Wtsi mice

Show the 2 phenotype image(s) involving this allele.

Mutation
origin
Mutant Cell Lines:  EPD0656_4_A03, EPD0656_4_A04, EPD0656_4_B03, EPD0656_4_B04, EPD0656_4_C03, EPD0656_4_D02, EPD0656_4_D04, EPD0656_4_E02, EPD0656_4_E03, EPD0656_4_F01, EPD0656_4_F02, EPD0656_4_F03, EPD0656_4_G01, EPD0656_4_H02, EPD0656_4_H04
Germline Transmission:  Earliest citation of germline transmission: J:250115
Parent Cell Line:  JM8A3.N1 (ES Cell)
Strain of Origin:  C57BL/6N-Atm1Brd
Project Collection: EUCOMM
Mutation
description
Allele Type:    Targeted (Conditional ready, Null/knockout, Reporter)
Mutation:    Insertion     Vector: L1L2_Bact_P
 
Mutation detailsThe L1L2_Bact_P cassette was inserted at position 77104991 of Chromosome 18 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 77105793. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534, J:250115)
Schematic of the Katnal2tm1a(EUCOMM)Wtsi knockout first, conditional-ready allele
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 1 line available
Carrying any Katnal2 Mutation:  201 strains or lines available
References
Original:  J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010;
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory