Podxl2^{tm1a(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Podxl2^{tm1a(EUCOMM)Wtsi}
• Name: podocalyxin-like 2; targeted mutation 1a, Wellcome Trust Sanger Institute
• MGI ID: MGI:4842467
• Gene: Podxl2 Location: Chr6:88819540-88852026 bp, - strand Genetic Position: Chr6, 39.6 cM
• Alliance: Podxl2^{tm1a(EUCOMM)Wtsi} page
• IMPC: Podxl2 gene page

Mutation origin

• Mutant Cell Lines: EPD0647_7_B05, EPD0647_7_C06, EPD0647_7_C08, EPD0647_7_E08, EPD0647_7_H07, EPD0647_7_H08
• Germline Transmission: Earliest citation of germline transmission: J:240523
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 88825445 of Chromosome 6 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 88827396. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:155845, J:173534, J:240523)

Expression

In Mice Carrying this Mutation:

10 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Podxl2 Mutation: 26 strains or lines available

Notes

Mutant cell line EPD0647_7_C06 was used to create mutant mice which demonstrated germline transmission (J:240523)

References

• Original: J:155845 Wellcome Trust Sanger Institute, Alleles produced for the EUCOMM and EUCOMMTools projects by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2010
• All: 3 reference(s)