Notch4^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Notch4^{tm1(KOMP)Vlcg}
• Name: notch 4; targeted mutation 1, Velocigene
• MGI ID: MGI:4842905
• Synonyms: Notch4^-
• Gene: Notch4 Location: Chr17:34783242-34807477 bp, + strand Genetic Position: Chr17, 18.15 cM
• Alliance: Notch4^{tm1(KOMP)Vlcg} page
• IMPC: Notch4 gene page

Mutation origin

• Mutant Cell Lines: 10800A-B2, 10800A-B7, 10800A-B11, 10800A-C3, 10800A-D2, 10800A-G2
• Germline Transmission: Earliest citation of germline transmission: J:204812
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 23547bp between positions 34783423-34806969 of Chromosome 17 (Build GRCm39). The entire coding region was replaced with a lacZ/neo cassette inserted between the start and stop codons, thus creating a null allele. Whole-mount immunofluorescence confirmed detection of anti-NOTCH4 extracellular domain reactivity in the vasculature of wild-type but not homozygous mutant P5 retinas. (J:136110, J:212472)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 1 line available
• Carrying any Notch4 Mutation: 87 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 3 reference(s)