Tmub1^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Tmub1^{tm1(KOMP)Vlcg}
• Name: transmembrane and ubiquitin-like domain containing 1; targeted mutation 1, Velocigene
• MGI ID: MGI:4842927
• Synonyms: Hops^-
• Gene: Tmub1 Location: Chr5:24650456-24652852 bp, - strand Genetic Position: Chr5, 11.79 cM, cytoband A3
• Alliance: Tmub1^{tm1(KOMP)Vlcg} page
• IMPC: Tmub1 gene page

Mutation origin

• Mutant Cell Lines: 11654A-D9, 11654A-E11, 11654C-B5, 11654C-B11, 11654C-D4, 11654C-F12, 11654C-G6
• Germline Transmission: Earliest citation of germline transmission: J:284575
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1
• Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 1027bp between positions 24650888-24651914 of Chromosome 5 (Build GRCm39). Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts isolated from homozygous mutant mice. (J:136110, J:284575)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Tmub1 Mutation: 23 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 2 reference(s)