Klhl40^{tm1(KOMP)Vlcg}
Targeted Allele Detail

Summary

• Symbol: Klhl40^{tm1(KOMP)Vlcg}
• Name: kelch-like 40; targeted mutation 1, Velocigene
• MGI ID: MGI:4843038
• Synonyms: Klhl40^-
• Gene: Klhl40 Location: Chr9:121606673-121612884 bp, + strand Genetic Position: Chr9, 72.61 cM, cytoband F4
• Alliance: Klhl40^{tm1(KOMP)Vlcg} page
• IMPC: Klhl40 gene page

Small size of P8 Klhl40^{tm1(KOMP)Vlcg}/Klhl40^{tm1(KOMP)Vlcg} pups

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Lines: 14780A-A4, 14780A-A7, 14780A-B6, 14780A-D10, 14780A-G10, 14780A-G12
• Germline Transmission: Earliest citation of germline transmission: J:213780
• Parent Cell Line: VGB6 (ES Cell)
• Strain of Origin: C57BL/6NTac
• Project Collection: KOMP-Regeneron

Mutation description

Allele Type: Targeted (Null/knockout, Reporter) Mutations: Insertion, Intragenic deletion Vector: ZEN-Ub1 Mutation details: The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 5645bp between positions 121606845-121612489 of Chromosome 9 (Build GRCm39). The coding sequence (excluding the start codon) as well as 23 nucleotides past the stop codon (nucleotides 172-5816 of NCBI reference sequence NC_000075.6) were replaced with a LacZ and neomycin expression cassette. The promoter and the 5' and 3' untranslated regions were left intact allowing for LacZ expression to be driven by the endogenous promoter. qPCR analysis confirmed absence of RNA transcript in quadriceps from P8 homozygous mutant mice. (J:136110, J:213780)

Generation of the Klhl40^tm1(KOMP)Vlcg allele

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Klhl40 Mutation: 26 strains or lines available

References

• Original: J:136110 Velocigene, Alleles produced for the KOMP project by Velocigene (Regeneron Pharmaceuticals). MGI Direct Data Submission. 2008
• All: 3 reference(s)