Ptcra^tm1.1Shoy
Targeted Allele Detail

Summary

• Symbol: Ptcra^tm1.1Shoy
• Name: pre T cell antigen receptor alpha; targeted mutation 1.1, Sho Yamasaki
• MGI ID: MGI:4843449
• Synonyms: pTalpha^4A
• Gene: Ptcra Location: Chr17:47066589-47074638 bp, - strand Genetic Position: Chr17, 22.9 cM, cytoband D-E1
• Alliance: Ptcra^tm1.1Shoy page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166147
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

Allele Type: Targeted (Inserted expressed sequence) Mutation: Insertion Mutation details: A floxed neomycin resistance cassette in reverse orientation was inserted in intron 1. Mutations were inserted in exon 2 causing an aspartic acid to alanine change at codon 22 (D22A) and arginine to alanine changes at codons 24 and 102 (R24A, R102A). An additional mutation in exon 3 changes codon 117 from arginine to alanine (R117A). All four mutated amino acids are in the extracellular domain of the protein. The selection cassette was subsequently removed by cre excision. (J:166147)

Generation of the Ptcra^tm1.1Shoy allele

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptcra Mutation: 16 strains or lines available

References

• Original: J:166147 Ishikawa E, et al., Germ-line elimination of electric charge on pre-T-cell receptor (TCR) impairs autonomous signaling for {beta}-selection and TCR repertoire formation. Proc Natl Acad Sci U S A. 2010 Nov 16;107(46):19979-84
• All: 1 reference(s)