Gls^{tm1a(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Gls^{tm1a(KOMP)Mbp}
• Name: glutaminase; targeted mutation 1a, Mouse Biology Program, UC Davis
• MGI ID: MGI:4843620
• Gene: Gls Location: Chr1:52202607-52272391 bp, - strand Genetic Position: Chr1, 26.86 cM
• Alliance: Gls^{tm1a(KOMP)Mbp} page
• IMPC: Gls gene page

Mutation origin

• Mutant Cell Lines: DEPD0005_8_A08, DEPD0005_8_A09, DEPD0005_8_A10, DEPD0005_8_A11, DEPD0005_8_A12, DEPD0005_8_B10, DEPD0005_8_C09, DEPD0005_8_C10, DEPD0005_8_D07, DEPD0005_8_D11, DEPD0005_8_D12, DEPD0005_8_E07, DEPD0005_8_E08, DEPD0005_8_E09, DEPD0005_8_E12, DEPD0005_8_F09, DEPD0005_8_G10, DEPD0005_8_G11, DEPD0005_8_G12, DEPD0005_8_H07, DEPD0005_8_H09
• Germline Transmission: Earliest citation of germline transmission: J:241505
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 52235077 of Chromosome 1 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 52236375. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064, J:321423)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 1 line available
• Carrying any Gls Mutation: 44 strains or lines available

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 6 reference(s)