Terb1^{tm1a(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Terb1^{tm1a(KOMP)Mbp}
• Name: telomere repeat binding bouquet formation protein 1; targeted mutation 1a, Mouse Biology Program, UC Davis
• MGI ID: MGI:4843634
• Gene: Terb1 Location: Chr8:105173351-105236542 bp, - strand Genetic Position: Chr8, 53.04 cM
• Alliance: Terb1^{tm1a(KOMP)Mbp} page
• IMPC: Terb1 gene page

Mutation origin

• Mutant Cell Lines: DEPD0002_1_A07, DEPD0002_1_A09, DEPD0002_1_B07, DEPD0002_1_B08, DEPD0002_1_B11, DEPD0002_1_C12, DEPD0002_1_D07, DEPD0002_1_D11, DEPD0002_1_E09, DEPD0002_1_E11, DEPD0002_1_E12, DEPD0002_1_F10, DEPD0002_1_F12, DEPD0002_1_G10, DEPD0002_1_G11, DEPD0002_1_G12, DEPD0002_1_H07, DEPD0002_1_H09, DEPD0002_1_H10, DEPD0002_1_H11
• Germline Transmission: Earliest citation of germline transmission: J:205574
• Parent Cell Line: JM8A1.N3 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 105208534 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 105209374. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:157064)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 2 lines available
• Carrying any Terb1 Mutation: 47 strains or lines available

Notes

Cell line DEPD0002_1_B08 was successfully used to make chimeric mice. Germline transmission was accomplished. J:205574

References

• Original: J:157064 University of California, Davis, Alleles produced for the KOMP project by the University of California, Davis. MGI Direct Data Submission. 2010
• All: 6 reference(s)