Hand1^{tm2(Hand1)Abfi}
Targeted Allele Detail

Summary

• Symbol: Hand1^{tm2(Hand1)Abfi}
• Name: heart and neural crest derivatives expressed 1; targeted mutation 2, Anthony B Firulli
• MGI ID: MGI:4843970
• Synonyms: Hand1^+Neo, Hand1^{tm1(Hand1)Abfi}
• Gene: Hand1 Location: Chr11:57719539-57722973 bp, - strand Genetic Position: Chr11, 35.27 cM
• Alliance: Hand1^{tm2(Hand1)Abfi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164028
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Mutation details: The coding region of Hand1 from a 600-bp cDNA initiating at the ATG and terminating at the stop codon followed by an EGFP expression cassette and a PGKNeo cassette flanked by loxP sites were inserted in frame at the position of the initiation codon. EGFP epifluorescence is not detected in E9.5 embryos, however EGFP mRNA is detected in a pattern identical to Hand1. qRT-PCR indicates that heterozygotes express about 90% of wild-type levels while homozygotes express at 50% that of wild-type, indicating that this is a hypomoprhic allele. In situ analysis indicates that expression is not uniformly reduced throughout the embryo. (J:164028)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hand1 Mutation: 15 strains or lines available

References

• Original: J:164028 Firulli BA, et al., Analysis of a Hand1 hypomorphic allele reveals a critical threshold for embryonic viability. Dev Dyn. 2010 Oct;239(10):2748-60
• All: 1 reference(s)