Hand1^{tm2.1(Hand1)Abfi}
Targeted Allele Detail

Summary

• Symbol: Hand1^{tm2.1(Hand1)Abfi}
• Name: heart and neural crest derivatives expressed 1; targeted mutation 2.1, Anthony B Firulli
• MGI ID: MGI:4843975
• Synonyms: Hand1^deltaNeo, Hand1^{tm1.1(Hand1)Abfi}
• Gene: Hand1 Location: Chr11:57719539-57722973 bp, - strand Genetic Position: Chr11, 35.27 cM
• Alliance: Hand1^{tm2.1(Hand1)Abfi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:164028
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: The coding region of Hand1 from a 600-bp cDNA initiating at the ATG and terminating at the stop codon followed by an EGFP expression cassette and a PGKNeo cassette flanked by loxP sites were inserted in frame at the position of the initiation codon. The PGKNeo cassette from Hand1^{tm2(Hand1)Abfi} was removed via cre-mediated recombination. EGFP expression is not detected in E9.5 embryos. qRT-PCR indicates that heterozygotes express about 50% of wild-type mRNA levels while homozygotes express at 30% that of wild-type, indicating that this is a hypomorphic allele. In situ analysis indicates that expression is not uniformly reduced throughout the embryo. (J:164028)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hand1 Mutation: 15 strains or lines available

References

• Original: J:164028 Firulli BA, et al., Analysis of a Hand1 hypomorphic allele reveals a critical threshold for embryonic viability. Dev Dyn. 2010 Oct;239(10):2748-60
• All: 1 reference(s)