F9^tm3Dws
Targeted Allele Detail

Summary

• Symbol: F9^tm3Dws
• Name: coagulation factor IX; targeted mutation 3, Darrel W Stafford
• MGI ID: MGI:4847605
• Synonyms: F9^tm2Dws, FIX^K5A
• Gene: F9 Location: ChrX:59044824-59076119 bp, + strand Genetic Position: ChrX, 33.5 cM, cytoband A6-A7
• Alliance: F9^tm3Dws page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:166332
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: In ES cells containing F9^tm1Dws, the neo cassette was replaced with sequence corresponding to the 5' Hprt minigene, exon 1, a modified exon 2, and exon 3. The modified exon 2 contains nucleotide substitutions that result in the amino acid substitution of alanine for lysine at position 5 (K5A). (J:166332)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any F9 Mutation: 8 strains or lines available

References

• Original: J:166332 Gui T, et al., Abnormal hemostasis in a knock-in mouse carrying a variant of factor IX with impaired binding to collagen type IV. J Thromb Haemost. 2009 Nov;7(11):1843-51
• All: 1 reference(s)